Lab Protocols

Internal protocols maintained on Google Drive.

Lab Tasks

• Task List

Molecular Biology

• General Lab Recipes

• Bacterial Freezer Stocks

• Bacterial Transformation

• Ethanol Precipitation

• DNA Ladder Creation

• Making Agarose Gels

• NEB HiFi Assembly

• qPCR

• cDNA Synthesis

• dsRNA Synthesis

• Competent Cells

Mosquito-Stage Parasite Protocols

• Insectary Operations

• Mosquito Blood Feeding

• Mosquito Carton Assembly

• L3 Emergence Assay

• L3 Extraction from Mosquitoes

• in squito RNAi

Parasitic Nematodes

Filarial nematodes

• Parasite Culture

• Dirofilaria immits L3 to L4 Molt

• Adult RNAi (Dialysis)

• RNA Extraction

• Cryoembedding

• Cryosectioning

• LCM

• Exosome Isolation

• Chemotaxis Assay

• Protein Release Assay

• Adult Phenotyping

• Microfilariae Purification

• Mansonella Diagnostics

GINs

• Egg Purification from Feces

• Modified McMaster's Fecal Egg Count

• H. contortus L3 Ex-sheathing

Caenorhabditis elegans

• C. elegans Recipes

• Chunking Worms

• Bleach Synchronization

• Cleaning Worms

• Extrachromosomal Integration

• Freezing Worms

• Freezing Worms in Agar

• RNA Extraction

• gDNA Extraction

• Single Worm Genotyping

• Plate Pouring: NGM/HGM plates

• Plate Pouring: Growing OP50

• Plate Pouring: Seeding plates

• Plate Pouring: Cholesterol

Phenotyping

• Stacks Sensory Assays

• Ring-based Aversion

• Aldicarb Paralysis

• Screenchip Electropharyngeogram (EPG) Recordings

• Pharyngeal Pump Counting

Microinjection

• C. elegans Microinjection

• Injection Rig Preparation

Schistosome Protocols

• Schistosome and Snail Recipes

• B. glabrata Maintenance

• Bge Cryopreservation

• Harvesting Miracidia

• B. glabrata Infections

Lab Operations

• Autoclave Operation

• Glass/Plastic Washing

• BSC Operation

• Liquid N₂ Tank Maintenance

• Water-jacketed Incubators

• Connecting CO₂ Tanks

General

• Label Maker

• Shipping Biomaterials

• Confocal Microscope

• Uploading NCBI Data

• Dissection Probe Assembly