Lab Protocols Lab Tasks Staff Tasks Undergraduate Tasks Molecular Biology General Lab Recipes Bacterial Freezer Stocks Bacterial Transformation Ethanol Precipitation DNA Ladder Creation Making Agarose Gels NEB HiFi Assembly Partial Digest qPCR cDNA Synthesis dsRNA Synthesis Sanger Sequencing Mosquito-Stage Parasite Protocols Insectary Operations Mosquito Blood Feeding Mosquito Carton Assembly L3 Extraction from Mosquitoes in squito RNAi Parasitic Nematodes Parasite Culture Dirofilaria immits L3 to L4 Molt Adult RNAi (Dialysis) RNA Extraction Cryoembedding Cryosectioning LCM Exosome Isolation Chemotaxis Assay Protein Release Assay Adult Phenotyping Caenorhabditis elegans Many protocols adapted from the Andersen Lab (NU) C. elegans Recipes Chunking Worms Bleach Synchronization Cleaning Worms Freezing Worms Freezing Worms in Agar RNA Extraction gDNA Extraction Single Worm Genotyping Plate Pouring: NGM/HGM plates Plate Pouring: Growing OP50 Plate Pouring: Seeding plates Plate Pouring: Cholesterol Microinjection C. elegans Microinjection Injection Rig Preparation Schistosome Protocols Schistosome and Snail Recipes B. glabrata Maintenance Bge Cryopreservation Harvesting Miracidia B. glabrata Infections Lab Operations Autoclave Operation Glass/Plastic Washing BSC Operation Liquid N2 Tank Maintenance Water-jacketed Incubators Connecting CO2 Tanks General Label Maker Shipping Biomaterials Confocal Microscope Uploading NCBI Data Dissection Probe Assembly