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Lab Protocols

High-Throughput Phenotyping (For internal use)

Lab Tasks

• Staff Tasks

• Undergraduate Tasks

Molecular Biology

• General Lab Recipes

• Bacterial Freezer Stocks

• Bacterial Transformation

• Ethanol Precipitation

• DNA Ladder Creation

• Making Agarose Gels

• NEB HiFi Assembly

• Partial Digest

• qPCR

• cDNA Synthesis

• dsRNA Synthesis

• Sanger Sequencing

Mosquito-Stage Parasite Protocols

• Insectary Operations

• Mosquito Blood Feeding

• Mosquito Carton Assembly

• L3 Emergence Assay

• L3 Extraction from Mosquitoes

• in squito RNAi

Parasitic Nematodes

Filarial nematodes

• Parasite Culture

• Dirofilaria immits L3 to L4 Molt

• Adult RNAi (Dialysis)

• RNA Extraction

• Cryoembedding

• Cryosectioning

• LCM

• Exosome Isolation

• Chemotaxis Assay

• Protein Release Assay

• Adult Phenotyping

• Microfilariae Purification

GINs

• Egg Purification from Feces

• Modified McMaster's Fecal Egg Count

Caenorhabditis elegans

Many protocols adapted from the Andersen Lab (NU)

• C. elegans Recipes

• Chunking Worms

• Bleach Synchronization

• Cleaning Worms

• Freezing Worms

• Freezing Worms in Agar

• RNA Extraction

• gDNA Extraction

• Single Worm Genotyping

• Plate Pouring: NGM/HGM plates

• Plate Pouring: Growing OP50

• Plate Pouring: Seeding plates

• Plate Pouring: Cholesterol

Microinjection

• C. elegans Microinjection

• Injection Rig Preparation

Schistosome Protocols

• Schistosome and Snail Recipes

• B. glabrata Maintenance

• Bge Cryopreservation

• Harvesting Miracidia

• B. glabrata Infections

Lab Operations

• Autoclave Operation

• Glass/Plastic Washing

• BSC Operation

• Liquid N₂ Tank Maintenance

• Water-jacketed Incubators

• Connecting CO₂ Tanks

General

• Label Maker

• Shipping Biomaterials

• Confocal Microscope

• Uploading NCBI Data

• Dissection Probe Assembly