Maintenance of Water-Jacketed Incubators
Important Reminders
-
Do NOT use bleach or any disinfectant that has high chlorine content.
-
Use sterile, distilled, or demineralized water.
-
Avoid spraying cleaner on the CO2 sensor.
-
Do not use powdered gloves for tissue cultures.
Maintenance Tasks
Action | Daily | Weekly | Monthly | 6 Months | Yearly | 2 Years |
---|---|---|---|---|---|---|
Inspect door latch hinges and door gasket seal | X | |||||
Check water level in humidity pan, ½" from top | X | |||||
Verify and document CO2, humidity, temp calibration | X | |||||
Perform complete decontamination procedure | As Needed | |||||
Change HEPA and gas filters | X | |||||
Replenish rust inhibitor in water jacket | X |
Filling humidity pan
-
Use sterilized, distilled, demineralized or deionized water. Water must be safe with stainless steel (no high purity, ultra pure or milli-q water). The pan is autoclavable.
-
Fill the pan to within ½ inch of the top with sterile, distilled water. The pan requires approximately 3 L of water to fill to within ½ inch of the top.
Calibration
See "Section 2: Calibration" in the manual.
Cleaning/decontamination
No bleach or any disinfectant with high chlorine content. See "Section 5: Routine Maintenance" in the manual for thorough cleaning instructions.
Changing HEPA and gas filters
See "Section 5-6" in the manual for instructions. An alarm can also be set to alert personnel when replacements need to occur (Section 5-6).
Rust inhibitor replenishment
-
Mix 1 bag/bottle of rust inhibitor with a gallon of distilled, demineralized, or deionized water.
-
Rust inhibitor (0.5 lb) bag (Part #: 1900100)
-
Rust inhibitor (800 mL) bottle (Part#:1900101)
-
Drain a gallon of water form the jacket and replace it with the rust inhibitor mixture.