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Schistosome and Snail Recipes

iBge Medium pH 7.2

Reagent Amount (per 100 mL)
Schneider's Drosophila Medium 22 mL
Lactalbumin hydrolysate 450 mg
Galactose 130 mg
Sterile DI water 68 mL
Gentamicin 50 mg/ml stock (Optional) 0.2 mL
  • Filter sterilize (0.22 um filter) and store at 4C

To prepare cBge medium, add 10 mL of heat inactivated FBS and 1 mL of 100X pen/strep to 89 mL iBge.

Note: iBge is very easily contaminated

Note: Do not freeze cBge Medium

Note: Bge cells are sensitive to the quality of FBS. Several lots of FBS may have to be screened. It has been known that investigators using Bge cell lines have soon lost it due to the unsuitability of their FBS.


Hansen's Buffered Saline pH 7.5-7.7

Reagent Amount (per 1 L)
NaCl 2.8 g
Sodium gluconate 1.5 g
KCl 0.15 g
Na2HPO4 0.07 g
HCO3 0.05 g
Glucose 0.5 g
Trehalose 0.5 g
Galactose 0.5 g
Sterile DI water 1 L

Chernin's Balanced Salt Solution (CBSS)

Reagent Amount (per 1 L)
NaCl 2.8 g
KCl 0.15 g
Na2HPO4 0.07 g
MgSO4 heptahydrate 0.45 g
CaCl2 dihydrate 0.53 g
NaHCO3 0.05 g
Sterile DI water 1 L
  • Filter sterilize (0.22 um filter) and store at 4C

  • To make CBSS+, add 1 g each of glucose and trehalose


Trypsin Solution 0.05%

Reagent Amount
Trypsin 50 mg
Hansen's buffered saline 100 mL
  • Filter sterilize (0.22 um filter).

  • Freeze in 10 mL aliquots.

  • Warm before using.


Pond Water Stock Solution

Reagent Amount (per 1 L)
CaCO3 12.5 g
MgCO3 1.25 g
NaCl 1.25 g
KCl 0.25 g
Sterile DI water 1 L
  • Pond water stocks are prepared in small Nalgene containers and stored on the supply rack in B7.

  • Shake the stock bottle well before diluting, as the reagents and salts may have settled on the bottom.

  • Common working Pond Water dilutions:

    • 100 mL Pond Water Stock → 30 Gallons dH2O

    • 33 ml Pond Water Stock → 10 Gallons dH2O

    • 16.5 mL Pond Water Stock → 5 Gallons dH2O

    • 12 ml Pond Water Stock → 10 Litres dH2O