Maintenance of Water-Jacketed Incubators
Do NOT use bleach or any disinfectant that has high chlorine content.
Use sterile, distilled, or demineralized water.
Avoid spraying cleaner on the CO2 sensor.
Do not use powdered gloves for tissue cultures.
|Action||Daily||Weekly||Monthly||6 Months||Yearly||2 Years|
|Inspect door latch hinges and door gasket seal||X|
|Check water level in humidity pan, ½" from top||X|
|Verify and document CO2, humidity, temp calibration||X|
|Perform complete decontamination procedure||As Needed|
|Change HEPA and gas filters||X|
|Replenish rust inhibitor in water jacket||X|
Filling humidity pan
Use sterilized, distilled, demineralized or deionized water. Water must be safe with stainless steel (no high purity, ultra pure or milli-q water). The pan is autoclavable.
Fill the pan to within ½ inch of the top with sterile, distilled water. The pan requires approximately 3 L of water to fill to within ½ inch of the top.
See "Section 2: Calibration" in the manual.
No bleach or any disinfectant with high chlorine content. See "Section 5: Routine Maintenance" in the manual for thorough cleaning instructions.
Changing HEPA and gas filters
See "Section 5-6" in the manual for instructions. An alarm can also be set to alert personnel when replacements need to occur (Section 5-6).
Rust inhibitor replenishment
Mix 1 bag/bottle of rust inhibitor with a gallon of distilled, demineralized, or deionized water.
Rust inhibitor (0.5 lb) bag (Part #: 1900100)
Rust inhibitor (800 mL) bottle (Part#:1900101)
Drain a gallon of water form the jacket and replace it with the rust inhibitor mixture.