BSC (Biosafety Cabinet) Operation
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Press the blower button to turn the BSC “on” and open the sash to the proper indicated level (red arrows on the side).
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Leave the sash open and the blower on for 5 minutes before beginning work to allow the BSC to purge any particulates. Keep blower on for the entire time the BSC is on and being used.
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Use 70% ethanol to disinfect the work surface of the BSC and all containers, equipment, etc. that is being used.
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When working in the BSC, move arms in and out slowly, make slow movements inside the cabinet as to not disrupt the airflow.
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Perform all operations at least 4 inches from the front grill on the work surface.
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The workflow inside the cabinet should be from “clean” materials to “dirty” (contaminated). Minimize movement of dirty items over clean ones.
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Anything going inside the BSC should be decontaminated with 70% ethanol.
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Any time gloves are taken out of the BSC, they must be sprayed with ethanol before going back inside.
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When finished, decontaminate all surfaces and materials with 70% ethanol, any waste should also be decontaminated and placed in biohazard bin.
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Close the sash, turn off the blower, then press and hold the UV light button for a few second until it beeps. This should turn on the UV light for ~20 minutes (be careful with the UV light when others are present in the room).