Preparation of Bacterial Freezer Stocks
Materials
- LB broth
- Cryogenic tubes
- 50% glycerol (autoclaved)
Protocol
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Pipette 5-10 mL LB (with appropriate antibiotic) into 15mL tube. For instructions on preparing LB broth, refer to Zamanian Lab Recipes.
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Inoculate a single colony from the streak or transformation plate.
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Set tube to shake in 37°C incubator overnight.
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After ~16 hours, take culture tubes out of the incubator and make freezer stocks as directed below.
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Label cryogenic tubes appropriately with a printed label.
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Add 500 μl of sterile 50% glycerol and an equal volume of culture to each cryo tube.
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Place tubes in correct -80°C and liquid N2 Bacterial Stock.
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Inventory freezer stocks in Quartzy.
See Lab Manual for complete tube labeling, tube count, and Quartzy inventory instructions.